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rabbit da1e mab igg xp isotype control  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit da1e mab igg xp isotype control
    Rabbit Da1e Mab Igg Xp Isotype Control, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/control+rabbit+igg/Rabbit+mAb+IgG+XP+Isotype+Control/pmc13010434-12-0-9
    Average 97 stars, based on 1279 article reviews
    rabbit da1e mab igg xp isotype control - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    Chromatin Immunoprecipitation:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Histone lactylation-mediated up-regulation of IGF2BP2 enhances ferroptosis resistance via Nrf2 in colorectal cancer.
    Article Snippet: .. Chromatin was isolated, fragmented using a micrococcal nuclease and immunoprecipitated overnight (4◦C) with ChIP-grade antibodies against H3K18la (22H2L3, PTM BIO), EP300 (33-7600; Thermo Fisher) or control rabbit IgG (Cat#2729; Cell Signaling Technology). ..

    Article Title: PP2A activation targets MYCN in neuroblastoma
    Article Snippet: .. After fragmenting the nuclear fraction via sonication (Fisherbrand Model 50 Sonic Dismembrator, ThermoFisher Scientific), the chromatin was incubated with anti-H3K27ac ChIP Grade antibody (2 μg per reaction, rabbit polyclonal, AB4729; Abcam Inc., Waltham, MA, USA) or control rabbit IgG (2729 P; Cell Signaling Technology) at 4 °C with rotation overnight, resuspended in ChIP-Grade Protein G beads to each immunoprecipitation reaction, and incubated for another 2 h at 4 °C with rotation. ..

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Incubation:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: PP2A activation targets MYCN in neuroblastoma
    Article Snippet: .. After fragmenting the nuclear fraction via sonication (Fisherbrand Model 50 Sonic Dismembrator, ThermoFisher Scientific), the chromatin was incubated with anti-H3K27ac ChIP Grade antibody (2 μg per reaction, rabbit polyclonal, AB4729; Abcam Inc., Waltham, MA, USA) or control rabbit IgG (2729 P; Cell Signaling Technology) at 4 °C with rotation overnight, resuspended in ChIP-Grade Protein G beads to each immunoprecipitation reaction, and incubated for another 2 h at 4 °C with rotation. ..

    Article Title: BRG1 exacerbates myocardial fibrosis after myocardial infarction by interacting with ZEB1
    Article Snippet: .. Equal amounts of protein lysates were incubated overnight at 4 °C with specific primary antibodies or control rabbit IgG (Cell Signaling Technology, China). .. Protein A/G magnetic beads (MedChemExpress, United States) were then added and incubated for 2 h at 4 °C.

    Article Title: Synthetic cancer-specific promoters
    Article Snippet: .. 60 μL blocked agarose beads were added to the supernatant fraction per reaction with control rabbit IgG, anti-c-Jun, or anti-FRA2 rabbit antibodies (purchased from CellSignaling) and incubated at 4° C. overnight with rotation. .. 40 μL of the diluted sample was kept aside as the input fraction before preclearing with non-blocked 75 μL ProteinA Agarose/Salmon Sperm DNA (50% Slurry) for 30 minutes at 4° C. with agitation.

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Control:

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation
    Article Snippet: A375 and RPMI-7951 cells were cross-linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20-163; Sigma-Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor ® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti-STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4°C. .. Subsequently, ChIP-Grade Protein A/G Magnetic Beads (cat. no. 16-663; Sigma-Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Article Title: Histone lactylation-mediated up-regulation of IGF2BP2 enhances ferroptosis resistance via Nrf2 in colorectal cancer.
    Article Snippet: .. Chromatin was isolated, fragmented using a micrococcal nuclease and immunoprecipitated overnight (4◦C) with ChIP-grade antibodies against H3K18la (22H2L3, PTM BIO), EP300 (33-7600; Thermo Fisher) or control rabbit IgG (Cat#2729; Cell Signaling Technology). ..

    Article Title: PP2A activation targets MYCN in neuroblastoma
    Article Snippet: .. After fragmenting the nuclear fraction via sonication (Fisherbrand Model 50 Sonic Dismembrator, ThermoFisher Scientific), the chromatin was incubated with anti-H3K27ac ChIP Grade antibody (2 μg per reaction, rabbit polyclonal, AB4729; Abcam Inc., Waltham, MA, USA) or control rabbit IgG (2729 P; Cell Signaling Technology) at 4 °C with rotation overnight, resuspended in ChIP-Grade Protein G beads to each immunoprecipitation reaction, and incubated for another 2 h at 4 °C with rotation. ..

    Article Title: BRG1 exacerbates myocardial fibrosis after myocardial infarction by interacting with ZEB1
    Article Snippet: .. Equal amounts of protein lysates were incubated overnight at 4 °C with specific primary antibodies or control rabbit IgG (Cell Signaling Technology, China). .. Protein A/G magnetic beads (MedChemExpress, United States) were then added and incubated for 2 h at 4 °C.

    Article Title: HDAC5 -encoded Microprotein NISM Mediates Nucleolar Formation and Ribosomal RNA Synthesis
    Article Snippet: DHX9 was detected using 1:500 anti-DHX9 antibody diluted in LiCOR blocking buffer, incubated at 4°C overnight with gentle shaking. .. IP of DHX9 was performed using 0.5 μg DHX9 antibody with the same conditions described previously, pre-clearing with 0.5 μg control Rabbit IgG (Cell Signaling, 2729). ..

    Article Title: Synthetic cancer-specific promoters
    Article Snippet: .. 60 μL blocked agarose beads were added to the supernatant fraction per reaction with control rabbit IgG, anti-c-Jun, or anti-FRA2 rabbit antibodies (purchased from CellSignaling) and incubated at 4° C. overnight with rotation. .. 40 μL of the diluted sample was kept aside as the input fraction before preclearing with non-blocked 75 μL ProteinA Agarose/Salmon Sperm DNA (50% Slurry) for 30 minutes at 4° C. with agitation.

    Article Title: STAT1 accelerates cutaneous melanoma progression through TUBB4A expression regulation.
    Article Snippet: A375 and RPMI‐7951 cells were cross‐linked with 1% formaldehyde (cat. no. 28908; Thermo Fisher Scientific, Inc.) and lysed using SDS Lysis Buffer (cat. no. 20‐163; Sigma‐Aldrich; Merck KGaA) and chromatin was fragmented by sonication using a Bioruptor® PICO (cat. no. B01080010 Diagenode SA). .. For each reaction, 100 μg chromatin lysate was diluted to a final volume of 500 μl with ChIP dilution buffer and incubated with 5 μg of anti‐STAT1 antibodies (1:100; cat. no. 14994; Cell Signaling Technology, Inc.) or control rabbit IgG (1:100; cat. no. 2729; Cell Signaling Technology, Inc.) overnight at 4 ̊C. .. Subsequently, ChIP‐Grade Protein A/G Magnetic Beads (cat. no. 16‐663; Sigma‐Aldrich; Merck KGaA) were added according to the manufacturer's protocol.

    Isolation:

    Article Title: Histone lactylation-mediated up-regulation of IGF2BP2 enhances ferroptosis resistance via Nrf2 in colorectal cancer.
    Article Snippet: .. Chromatin was isolated, fragmented using a micrococcal nuclease and immunoprecipitated overnight (4◦C) with ChIP-grade antibodies against H3K18la (22H2L3, PTM BIO), EP300 (33-7600; Thermo Fisher) or control rabbit IgG (Cat#2729; Cell Signaling Technology). ..

    Immunoprecipitation:

    Article Title: Histone lactylation-mediated up-regulation of IGF2BP2 enhances ferroptosis resistance via Nrf2 in colorectal cancer.
    Article Snippet: .. Chromatin was isolated, fragmented using a micrococcal nuclease and immunoprecipitated overnight (4◦C) with ChIP-grade antibodies against H3K18la (22H2L3, PTM BIO), EP300 (33-7600; Thermo Fisher) or control rabbit IgG (Cat#2729; Cell Signaling Technology). ..

    Article Title: PP2A activation targets MYCN in neuroblastoma
    Article Snippet: .. After fragmenting the nuclear fraction via sonication (Fisherbrand Model 50 Sonic Dismembrator, ThermoFisher Scientific), the chromatin was incubated with anti-H3K27ac ChIP Grade antibody (2 μg per reaction, rabbit polyclonal, AB4729; Abcam Inc., Waltham, MA, USA) or control rabbit IgG (2729 P; Cell Signaling Technology) at 4 °C with rotation overnight, resuspended in ChIP-Grade Protein G beads to each immunoprecipitation reaction, and incubated for another 2 h at 4 °C with rotation. ..

    Sonication:

    Article Title: PP2A activation targets MYCN in neuroblastoma
    Article Snippet: .. After fragmenting the nuclear fraction via sonication (Fisherbrand Model 50 Sonic Dismembrator, ThermoFisher Scientific), the chromatin was incubated with anti-H3K27ac ChIP Grade antibody (2 μg per reaction, rabbit polyclonal, AB4729; Abcam Inc., Waltham, MA, USA) or control rabbit IgG (2729 P; Cell Signaling Technology) at 4 °C with rotation overnight, resuspended in ChIP-Grade Protein G beads to each immunoprecipitation reaction, and incubated for another 2 h at 4 °C with rotation. ..



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